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KMID : 1007520230320050689
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Food Science and Biotechnology
2023 Volume.32 No. 5 p.689 ~ p.696
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Synthesis of hexyl butyrate (apple and citrus aroma) by Candida rugosa lipase immobilized on Diaion HP-20 using the Box-Behnken design
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Marta Maria Oliveira dos Santos
Luiz Henrique Sales de Menezes
Eliezer Luz do Espirito Santo
Marise Silva de Carvalho
Marcia Soares Goncalves
Iasnaia Maria de Carvalho Tavares
Adriano Aguiar Mendes
Lee Chang-Youl
Luiz Carlos Salay
Marcelo Franco
Julieta Rangel de Oliveira
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Abstract
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This study aims at the synthesis of hexyl butyrate by Candida rugosa lipase (CRL) immobilized on Diaion HP 20. The lipase load used was 28.7?±?2.1 mg/g (mg of lipase/g of support), whose hydrolytic activity was 132.0?±?2.5 U/g. To obtain the maximum production of hexyl butyrate, the Box-Behnken design statistical planning was used, having as independent variables; biocatalyst concentration, temperature and acid:alcohol molar ratio and ester conversion as a dependent variable at 60, 180 and 480 min. For 60 min, 90.8% conversion was obtained at 47.25 ºC, 1:1.4 molar ratio and 17.65% of biocatalyst; 180 min, 94.5% conversion at 59.5 ºC, 1:2 molar ratio and 15.8% biocatalyst; 480 min, 95.01% conversion at 47.0 ºC, 1:2 molar ratio and 16.9% biocatalyst. CRL-Diaion HP 20 retained 60% of its initial activity after ten cycles of reactions showing potential for industrial use. The ester produced was identified by gas chromatography analyses.
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KEYWORD
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Diaion HP-20, Enzyme, Immobilization, Esterification
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